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View Full Version : NYC Bone Seminar 12/12/01 on FLUID FLOW EFFECTS ON BONE CELLS:INFLUENCE OF FLOW-CELL-SUBSTRATE; INTERACTIONS AND CELLMECHANICAL PROPERTIES



Steve Cowin
12-06-2001, 05:08 AM
To Bone Researchers in the NYC area:
The NYC mineralized tissue seminar will have its fourth
seminar in its new millennium series on Wednesday night December
12th. This is the last seminar in the fall 2001 series. The speaker
is Clark T. Hung PhD, Assistant Professor of Biomedical Engineering,
Cellular Engineering Laboratory, Cardiac Cell Mechanics Laboratory
and Bone Bioengineering Laboratory, Department of Biomedical
Engineering, Columbia University, New York, NY 10027. He will speak
on: FLUID FLOW EFFECTS ON BONE CELLS: INFLUENCE OF
FLOW-CELL-SUBSTRATE; INTERACTIONS AND CELL MECHANICAL PROPERTIES. An
abstract of this talk and a description of his research interests are
given below. The same information on the coming spring 2002 seminar
speakers will be posted in January 2002 on www.bonenet.net and will
be circulated as the date of each seminar approaches.

Speaker: Clark T. Hung PhD, Assistant Professor of Biomedical
Engineering, Cellular Engineering Laboratory, Cardiac Cell Mechanics
Laboratory and Bone Bioengineering Laboratory, Department of
Biomedical Engineering, Columbia University, New York, NY 10027

Title: FLUID FLOW EFFECTS ON BONE CELLS: INFLUENCE OF
FLOW-CELL-SUBSTRATE; INTERACTIONS AND CELL MECHANICAL PROPERTIES

Abstract: Bone cell mechanotransduction studies have focused more
recently on fluid flow related stimuli. Using parallel-plate or
laminar flow chambers, a well-defined stimulus can be applied to
cultured cells while permitting optical microscopy, biochemical and
molecular assays to be performed. The flow of fluid over the cells
gives rise to several potential concomitant stimuli including a
hydrostatic pressure gradient, convective transport of agonists,
electrokinetic phenomena (e.g., streaming potentials), and
fluid-induced shear stress. We have initiated several bioengineering
studies to better understand the role of fluid-induced shear stress
and related cell deformation in this in vitro model system. Numerical
modeling of fluid flow over hemispherical deformable cells on a flat
plane demonstrate that the shear levels that are "seen" by the cell
are several-fold greater than that described by the macroscopic wall
shear stress, the parameter typically used to describe the applied
fluid stimulus. However, these calculations are dependent on the
material properties of the cell as well as assumptions regarding
cell-substrate interactions. Accordingly, studies are underway to
include a triphasic model of the cell (treating the cell as a fluid,
solid and anion/cation phases). Parallel studies using an atomic
force microscope will permit an independent method to assess cell
properties on various biological substrates to provide inputs for
this triphasic cell model. Lastly, we have also undertaken bone cell
adhesion and biochemical studies to gain a further understanding of
cell-substrate interactions and signaling that may participate in the
bone cell response to fluid flow and correlate these findings to our
modeling studies and cell properties obtained by AFM. This is joint
work with Kevin D. Costa and X. Edward Guo

RESEARCH INTERESTS OF CLARK HUNG: Clark is interested in physical
effects on cells and orthopaedic cellular and tissue engineering.

WHERE AND WHEN: The seminar series is to be held this Fall in room
3309 at the City University of New York (CUNY) Graduate Center (GC)
on Wednesdays from 7 to 8:30 PM. There will be some socializing
before the seminar in the GC snack bar on the first floor, besides
the usual snacks and drinks the 365 Express also carries beer and
some other alcoholic beverages.

TRAVEL TO THE CITY UNIVERSITY OF NEW YORK (CUNY) GRADUATE CENTER (GC)

The Graduate Center (GC) of the City University of New York
(CUNY) is located in the newly renovated Altman Building at 365 5th
Avenue. The Altman Building occupies a city block bounded by 35th and
34th street on the north and south, respectively, and by Madison
Avenue and 5th Avenue and on the east and west, respectively. The
Altman Building is catty-corner from the Empire State Building. The
GC shares this building with the Science Division of the New York
Public Library and Oxford University Press. The entrance to the GC is
on 5th Avenue between, and almost equidistant from, East 34th Street
and East 35th Street.

The Pennsylvania Station (Amtrak, LLRR and New Jersey
Transit) is between 31st street and 33rd street on 7th Avenue. Grand
Central Station (Metro North) is on 42nd Street at Park Avenue. Park
Avenue is two blocks east of 5th Avenue (Madison is in between). Both
of these main line stations are short walks from the GC. The PATH
trains to New Jersey have a station at 34th Street and 6th Avenue.
There is also a heliport at 34th Street and East river, 6 avenue
blocks to the east.

Almost all the Manhattan subway lines have a station on 34th
Street and within several avenue blocks of the GC. There are a number
of bus lines that run on 34th street and on 5th Avenue. There are a
number of parking garages around the GC. There is money to support
parking for interested students, apply to Steve Cowin
(scccc@cunyvm.cuny,edu).

THE LOCATION OF THE BONE SEMINAR ACTIVITIES WITHIN THE GRADUATE CENTER (GC)

When you enter the GC from 5th Avenue there is a reception
counter in the center of a reception room and the elevators are
straight ahead. If you arrive at the GC with a few minutes to spare
before 7 PM you should consider stopping at the 365 Express on the
1st floor for sandwiches, snacks and drinks and to socialize with the
other seminar attendees and the evening's speaker. The 365 Express is
directly off the GC reception room on the 1st floor (the room you
entered from the street), off the southeast corner of the room. The
seminar room is on the 3rd floor (#3309) and accessible by elevator.
Before you are allowed to walk down the hall to the bank of elevators
you must identify yourself to the security people. This can be done
with a CUNY ID or a picture ID (and your signature in a guest book).
When you get off the elevator on the 3rd floor you head east, then
north, then east again to arrive at room 3309.

CLOSING NOTES

Future seminars are posted on www.bonenet.net, a website dedicated to
research on the mechanosensory system in bone. (This website is
operational, but not fully developed and suggestions for further
development of the site would be appreciated.) You may request a
reminder for each seminar by sending an email to Steve Cowin
(scccc@cunyvm.cuny.edu).

We will welcome your attendance and hope that you will pass along
this information to interested colleagues. Please direct your
questions, requests for more information and feedback to me.

Steve Cowin
--

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Stephen C. Cowin
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New York, NY 10024

Phone (212) 799-7970 (Office at Home)
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WORK ADDRESS:
Stephen C. Cowin
Director, New York Center for Biomedical Engineering
School of Engineering
The City College
138th Street and Convent Avenue
New York, NY 10031-9198, U. S. A.
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