View Full Version : tendon TEM protocol

Lou Soslowsky
10-15-1993, 07:34 AM
REGARDING tendon TEM protocol
To: Biomch-L community

I am interested to hear from anyone who has used transmission electron
microscopy to look at cross-sections of tendon. I am working on a project that
is attempting to make measures of fibril diameters and distributions. I
believe that I need help with protocols for fixation and embedding. I have
tried several variations of the usual methods and have gotten less than
satisfactory results. I am aware of some work by Parry and by Oakes in this
area but would be interested to "speak" (e-mail) with someone about the issues
and protocols to get some expert advice on my problem. Any help would be
greatly appreciated. Thanks in advance.

Lou Soslowsky, PhD
University of Michigan