View Full Version : Stereology Responses

Karl J. Jepsen
10-07-1996, 09:52 PM
Here is a listing of the responses I received regarding the stereology
software. Thanks to all.

Karl J. Jepsen, Ph.D.
Department of Orthopaedics
Case Western Reserve University
11100 Euclid Ave.
Cleveland, OH 44106


We have recently used a SEM based stereo imaging software to quantify
the surface roughness of human and bovine cortical bone. The preliminary
results are presented in form the following abstract :

Vashishth, D. and Bonfield, W., "Aetiology of cortical bone fracture",10th
conference of the European Society of Biomechanics,Leuven, August 28-31,
1996, pg. 55.

The sotware was supplied by Oxford instruments, Analytical systems division,
(Halifax road, High Wycombe, Bucks,England HP 12 3 SE) and it works in
conjunction with a link system (an imaging/analysis system for SEM images;
manufactured by Oxford instruments). If you need any further details, please
let me know.

With best wishes

Deepak Vashishth
IRC in Biomedical Materials
Queen Mary and Westfield College
University of London, UK

Hi Karl,
Maria Moalli was showing me a brochure recently from a company called R&M
Biometrics about a PC program called 'Bioquant', which is capable of doing
stereology with biological images. Maria is planning on attending a program
demo here at UofM in the end of October. If you want more info, I can xerox
the brochures and mail or fax (about 20 pages) them to you.

One more thing - The Bioquant brochure indicates that the program requires
an 'add-on' stereology toolkit module in order to use it for this

Take care,

Colleen L. Flanagan
Orthopaedic Research Lab
University of Michigan

For image processing and analysis, we use a commercial package called
Image-Pro Plus (V1.3) by Media Cybernetics. It has several available
enhancements and an SDK so you can add your own enhancements. We use it
to measure area fractions of bone and fiber metal in backscatter SEM for
uncemented hip replacements. We also use it for cortical porosity
measurements. We just bought it, so I really think we have barely
exploited all its potentials.

We got it because we thought our old system might kick the bucket. Our
old system is a hardware-based Image Technology Corp Model 3000. Its
software side runs in DOS. I don't know if that company still exists,
and if so, whether they have a software-based image analysis package.
You have to feed their black box a video image from somewhere. We use a
Data Translation DT2851-60 for this purpose and also for camera
acquisition. An EE masters student wrote a program called Cancellous
Bone Architecture or CBA. CBA can give us such measurements as bone area,
bone area density, bone perimeter, intertrabecular area, trabecular
width, etc. Unfortunately, it's still part of our legacy systems. It's a
text-mode program, but can be made to run in Windows (same with ITC
3000). But it's main drawback is it's progammed to use the DT2851-60,
which prevents us from using any computer with 16M of RAM or greater.


Ruben V. Igloria, Jr., Engineering Assistant
Orthopedic Research, Dept of Orthopedic Surgery
Rush Arthritis and Orthopedics Institute
Rush-Presbyterian-St. Luke's Medical Center
2242 W. Harrison St., Suite 103, Chicago, IL 60612-3515