PDA

View Full Version : Cytotoxic Agents



Alex Kerin
02-17-2000, 01:21 AM
Hi,

I am conducting some research on articular cartilage tissue explants. I
need to kill the chondrocytes, or at least stop them producing matrix
macromolecules, without affecting the mechanical properties of the
tissue. I would prefer not to use freeze-thaw cycles as I am unconvinced
that properties will not change (although if nothing else is suggested,
I will have to do before and after freeze-thaw controls). It has been
suggested that I use Sodium Azide, but I have found no information on
concentration/incubation time to acheive cell death. If anyone has any
suggestions that would be great.

Regards, Alex Kerin
--


Center for Biomedical Engineering
Rm 38-377, M.I.T.
77 Massachusetts Avenue
Cambridge, MA 02139
U.S.A.

Tel: + (617) 253 6944
Fax: + (617) 258 5239
Email: akerin@mit.edu

---------------------------------------------------------------
To unsubscribe send SIGNOFF BIOMCH-L to LISTSERV@nic.surfnet.nl
For information and archives: http://isb.ri.ccf.org/biomch-l
---------------------------------------------------------------