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cell alignment image analysis

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  • cell alignment image analysis

    Date: Monday, November 30, 1992
    To: Ed Grood, interested Biomch-L readers
    From: Joe Sommer, Penn State/NIH
    Subj: cell alignment image analysis
    Ref: Biomch-L posting 11/11/92
    "Response of fibroblasts to mechanical deformation"

    1) While a 2D FFT can provide overall image angular orientation
    information, I do not believe that it can provide angular
    DISTRIBUTION information such as you desire. This is based on
    personal experience in 2D FFT image analysis of quarry faces to
    assess rock fracture spacing/orientation for mining safety, and
    of polyurethane foam pore size/orientation for manufacturing
    quality control.

    2) If the cells are elongated, a linear Hough transform may be
    able to provide orientation distribution information. The Hough
    transform is a histogram method that characterizes straight lines
    in a 2D image by their slope angle THETA and their common
    perpendicular from the origin RHO, and produces a new image in
    the (RHO,THETA) domain where pixel intensity codes length of
    individual line segments with that slope slope and offset.
    Collapsing the 2D Hough image along the RHO axis after suitable
    thresholding, may be able to give an idea of the orientation

    3) Without seeing a sample image, I am hesitant to recommend
    automated image enhancement/segmentation methods "to separate
    overlapping cells, remove dirt objects, etc.", in that my
    experience has shown these tasks to be highly application
    specific. If you can prepare a sample image for Internet
    transfer, I would be happy to correspond further and perhaps even
    run a sample 2D FFT or Hough for you.

    Best wishes,
    Joe Sommer

    H.J. Sommer III, Professor of Mechanical Engineering, 207 Reber Building
    The Pennsylvania State University, University Park, PA 16802
    (814)865-2519, FAX (814)863-4848, Bitnet HJS@PSUECL, Internet HJS@ECL.PSU.EDU

    SABBATICAL ADDRESS (8/15/92-6/15/93)
    H.J. Sommer III, Guest Researcher, Biomechanics Lab, Rehabilitation Medicine
    National Institutes of Health, Bldg 10, Room 6s-207a, Bethesda, MD 20892
    (301)496-9890x15, FAX (301)402-0663, Internet SOMMER%BMLVAX.DNET@DXI.NIH.GOV