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To Bone Researchers in the NYC area:
There are changes, but the NYC mineralized tissue seminar
will still have its first seminar in the Spring 2002 series on
Thursday night, January 31st. The speaker and the room have been
changed from the announcement three days ago due to the flu and a
miscommunication. The speaker is now is Michael Hadjiargyrou,
Professor of Orthopaedics Assistant Professor of Biomedical
Engineering, Orthopaedics and Genetics, Department of Biomedical
Engineering, State University of New York, Stony Brook, NY. He will
speak on TRANSCRIPTIONAL PROFILING OF THE EARLY FRACTURE CALLUS: A
KEY TO BONE BIOENGINEERING? An abstract of this talk and a
description of Michael's research interests are given below. The same
information on the other spring seminar speakers will soon be posted
on www.bonenet.net. The room is now C197 rather than 9207.
Speaker: Michael Hadjiargyrou, Ph.D. Assistant Professor of
Biomedical Engineering, Orthopaedics and Genetics, Department of
Biomedical Engineering, State University of New York, Stony Brook, NY.
Title: TRANSCRIPTIONAL PROFILING OF THE EARLY FRACTURE CALLUS: A KEY
TO BONE BIOENGINEERING?
Abstract: Bone regeneration occurs as an elaborate series of events
that requires temporal and spatial orchestration of numerous cell
types and expression of hundreds to thousands of genes. The healing
of a fractured bone is, in essence, a recapitulation of embryonic
bone development that proceeds through similar processes such as
chondrogenesis, ossification and remodeling. In order to be able to
influence these biological events and thus the overall bone
regeneration process, a more comprehensive molecular understanding is
essential. In an effort to identify gene expression patterns that
occur during bone regeneration, a cDNA library was constructed. This
library consisted of transcriptionally induced genes (pooled from RNA
isolated from post fracture (PF) 3, 5, 7 and 10 day callus) that were
subtracted following hybridization with RNA derived from intact bone.
Following amplification, subtractive hybridization and cloning, 4,183
cDNA clones were identified as up-regulated genes and further
characterized. Of these, 3,799 (91%) were successfully sequenced.
These genes included 301 (8%) and 60 (1.6%) that showed homology to
mitochondrial and ribosomal genes, respectively. In addition, 2,002
(52.7%) had homology to other known genes and represented multiple
functional gene families. Further, more than one third of these
clones had no functional information in the literature or public
databases. Of these, 1,317 (34.7%) showed homology to expressed
sequenced tags (EST's) and 119 (3%) were completely novel. To obtain
a more comprehensive understanding of temporal gene expression and
significance of the genes in the healing process, custom microarrays
were constructed that contained all 4,183 clones. PF day 3, 5, 7,
10, 14 and 21 callus RNA samples were used to probe these microarrays
and confirm that greater than 80% of cDNAs are up-regulated greater
than two fold, on at least one of the PF days, in comparison with
intact bone. We are currently investigating the differential
expression of these genes as a function of time (i.e. progression of
the healing callus), and performing cluster analysis to potentially
assign function to the thousands of EST's, novel sequences and known
genes that have not as yet been described as involved in the bone
regeneration process. Taken together, these data provide a "window"
into the molecular events responsible for the early phases of bone
regeneration and suggest that many of the genes involved remain
uncharacterized.
RESEARCH INTERESTS OF MICHAEL HADJIARGYROU: Major research efforts
are directed towards understanding the molecular mechanisms that
underlie the wound healing (i.e. fracture repair) process, as well as
normal bone development. Related areas of interest extend to
biomaterials, gene therapy and tissue engineering.
NOTE: BEFORE MICHAEL HADJIARGYROU SPEAKS, TIM BROMAGE (ANTHROPOLOGY,
HUNTER COLLEGE) WILL TAKE ABOUT 2 OR 3 MINUTES TO PRESENT EXCERPTS
FROM AN EXHIBIT OF DIGITAL PHOTOMICROGRAPHY OF HARD TISSUES THAT HE
CURATED. THE EXHIBIT IS ENTITLED "THE MICROSCOPE AND THE SKELETON"
AND IT WILL OPEN AT HUNTER COLLAGE TWO DAYS BEFORE THE NEXT SEMINAR,
ON TUESDAY FEBRUARY 19TH. SEE BONENET.NET FOR MORE DETAILS; CLICK ON
THE PICTURE ON THE FIRST PAGE. THERE IS ONE WORD THAT IS INCORRECT ON
THE WEBSITE POSTING, OPENING RECEPTION: FRIDAY, FEBRUARY 19,
5:30-7:30 P.M. SHOULD BE OPENING RECEPTION: TUESDAY, FEBRUARY 19,
5:30-7:30 P.M.
WHERE AND WHEN: The seminar series is to be held this Spring in room
C197 (except for the March seminar which will be in Room 9207) at the
City University of New York (CUNY) Graduate Center (GC) on Thursdays
from 7 to 8:30 PM. The room C197 is in the concourse in the cellar).
There will be some socializing before the seminar in the GC snack bar
on the first floor, the 365 Express.
TRAVEL TO THE CITY UNIVERSITY OF NEW YORK (CUNY) GRADUATE CENTER (GC)
The Graduate Center (GC) of the City University of New York
(CUNY) is located in the newly renovated Altman Building at 365 5th
Avenue. The Altman Building occupies a city block bounded by 35th and
34th street on the north and south, respectively, and by Madison
Avenue and 5th Avenue and on the east and west, respectively. The
Altman Building is catty-corner from the Empire State Building. The
GC shares this building with the Science Division of the New York
Public Library and Oxford University Press. The entrance to the GC is
on 5th Avenue between, and almost equidistant from, East 34th Street
and East 35th Street.
The Pennsylvania Station (Amtrak, LLRR and New Jersey
Transit) is between 31st street and 33rd street on 7th Avenue. Grand
Central Station (Metro North) is on 42nd Street at Park Avenue. Park
Avenue is two blocks east of 5th Avenue (Madison is in between). Both
of these main line stations are short walks from the GC. The PATH
trains to New Jersey have a station at 34th Street and 6th Avenue.
There is also a heliport at 34th Street and East river, 6 avenue
blocks to the east.
Almost all the Manhattan subway lines have a station on 34th
Street and within several avenue blocks of the GC. There are a number
of bus lines that run on 34th street and on 5th Avenue. There are a
number of parking garages around the GC. There is money to support
parking for interested students, apply to Steve Cowin
(scccc@cunyvm.cuny,edu).
THE LOCATION OF THE BONE SEMINAR ACTIVITIES WITHIN THE GRADUATE CENTER (GC)
When you enter the GC from 5th Avenue there is a reception
counter in the center of a reception room and the elevators are
straight ahead. If you arrive at the GC with a few minutes to spare
before 7 PM you should consider stopping at the 365 Express on the
1st floor for sandwiches, snacks and drinks and to socialize with the
other seminar attendees and the evening's speaker. The 365 Express is
directly off the GC reception room on the 1st floor (the room you
entered from the street), off the southeast corner of the room. Room
C197 is in the cellar concourse. (For the March seminar, Room 9207 is
on the 9th floor.)
CLOSING NOTES
Future seminars are posted on www.bonenet.net, a website dedicated to
research on the mechanosensory system in bone. (This website is
operational, but not fully developed and suggestions for further
development of the site would be appreciated.) You may request a
reminder for each seminar by sending an email to Steve Cowin
(scccc@cunyvm.cuny.edu).
We will welcome your attendance and hope that you will pass along
this information to interested colleagues. Please direct your
questions, requests for more information and feedback to me.
Kind regards, Steve Cowin
--
************************************
For bone research information, visit .
************************************
PREFERRED MAILING ADDRESS
Stephen C. Cowin
2166 Broadway
Apartment 12D
New York, NY 10024
Phone (212) 799-7970 (Office at Home)
Fax (212) 799-7970 (Office at Home)
Phone (212) 650-5208 (Work)
Fax (212) 650-6727 (Work)
Email
WORK ADDRESS:
Stephen C. Cowin
Director, New York Center for Biomedical Engineering
School of Engineering
The City College
138th Street and Convent Avenue
New York, NY 10031-9198, U. S. A.
*************************************
For information about the New York Center for Biomedical
Engineering visit
*************************************
---------------------------------------------------------------
To unsubscribe send SIGNOFF BIOMCH-L to LISTSERV@nic.surfnet.nl
For information and archives: http://isb.ri.ccf.org/biomch-l
---------------------------------------------------------------
There are changes, but the NYC mineralized tissue seminar
will still have its first seminar in the Spring 2002 series on
Thursday night, January 31st. The speaker and the room have been
changed from the announcement three days ago due to the flu and a
miscommunication. The speaker is now is Michael Hadjiargyrou,
Professor of Orthopaedics Assistant Professor of Biomedical
Engineering, Orthopaedics and Genetics, Department of Biomedical
Engineering, State University of New York, Stony Brook, NY. He will
speak on TRANSCRIPTIONAL PROFILING OF THE EARLY FRACTURE CALLUS: A
KEY TO BONE BIOENGINEERING? An abstract of this talk and a
description of Michael's research interests are given below. The same
information on the other spring seminar speakers will soon be posted
on www.bonenet.net. The room is now C197 rather than 9207.
Speaker: Michael Hadjiargyrou, Ph.D. Assistant Professor of
Biomedical Engineering, Orthopaedics and Genetics, Department of
Biomedical Engineering, State University of New York, Stony Brook, NY.
Title: TRANSCRIPTIONAL PROFILING OF THE EARLY FRACTURE CALLUS: A KEY
TO BONE BIOENGINEERING?
Abstract: Bone regeneration occurs as an elaborate series of events
that requires temporal and spatial orchestration of numerous cell
types and expression of hundreds to thousands of genes. The healing
of a fractured bone is, in essence, a recapitulation of embryonic
bone development that proceeds through similar processes such as
chondrogenesis, ossification and remodeling. In order to be able to
influence these biological events and thus the overall bone
regeneration process, a more comprehensive molecular understanding is
essential. In an effort to identify gene expression patterns that
occur during bone regeneration, a cDNA library was constructed. This
library consisted of transcriptionally induced genes (pooled from RNA
isolated from post fracture (PF) 3, 5, 7 and 10 day callus) that were
subtracted following hybridization with RNA derived from intact bone.
Following amplification, subtractive hybridization and cloning, 4,183
cDNA clones were identified as up-regulated genes and further
characterized. Of these, 3,799 (91%) were successfully sequenced.
These genes included 301 (8%) and 60 (1.6%) that showed homology to
mitochondrial and ribosomal genes, respectively. In addition, 2,002
(52.7%) had homology to other known genes and represented multiple
functional gene families. Further, more than one third of these
clones had no functional information in the literature or public
databases. Of these, 1,317 (34.7%) showed homology to expressed
sequenced tags (EST's) and 119 (3%) were completely novel. To obtain
a more comprehensive understanding of temporal gene expression and
significance of the genes in the healing process, custom microarrays
were constructed that contained all 4,183 clones. PF day 3, 5, 7,
10, 14 and 21 callus RNA samples were used to probe these microarrays
and confirm that greater than 80% of cDNAs are up-regulated greater
than two fold, on at least one of the PF days, in comparison with
intact bone. We are currently investigating the differential
expression of these genes as a function of time (i.e. progression of
the healing callus), and performing cluster analysis to potentially
assign function to the thousands of EST's, novel sequences and known
genes that have not as yet been described as involved in the bone
regeneration process. Taken together, these data provide a "window"
into the molecular events responsible for the early phases of bone
regeneration and suggest that many of the genes involved remain
uncharacterized.
RESEARCH INTERESTS OF MICHAEL HADJIARGYROU: Major research efforts
are directed towards understanding the molecular mechanisms that
underlie the wound healing (i.e. fracture repair) process, as well as
normal bone development. Related areas of interest extend to
biomaterials, gene therapy and tissue engineering.
NOTE: BEFORE MICHAEL HADJIARGYROU SPEAKS, TIM BROMAGE (ANTHROPOLOGY,
HUNTER COLLEGE) WILL TAKE ABOUT 2 OR 3 MINUTES TO PRESENT EXCERPTS
FROM AN EXHIBIT OF DIGITAL PHOTOMICROGRAPHY OF HARD TISSUES THAT HE
CURATED. THE EXHIBIT IS ENTITLED "THE MICROSCOPE AND THE SKELETON"
AND IT WILL OPEN AT HUNTER COLLAGE TWO DAYS BEFORE THE NEXT SEMINAR,
ON TUESDAY FEBRUARY 19TH. SEE BONENET.NET FOR MORE DETAILS; CLICK ON
THE PICTURE ON THE FIRST PAGE. THERE IS ONE WORD THAT IS INCORRECT ON
THE WEBSITE POSTING, OPENING RECEPTION: FRIDAY, FEBRUARY 19,
5:30-7:30 P.M. SHOULD BE OPENING RECEPTION: TUESDAY, FEBRUARY 19,
5:30-7:30 P.M.
WHERE AND WHEN: The seminar series is to be held this Spring in room
C197 (except for the March seminar which will be in Room 9207) at the
City University of New York (CUNY) Graduate Center (GC) on Thursdays
from 7 to 8:30 PM. The room C197 is in the concourse in the cellar).
There will be some socializing before the seminar in the GC snack bar
on the first floor, the 365 Express.
TRAVEL TO THE CITY UNIVERSITY OF NEW YORK (CUNY) GRADUATE CENTER (GC)
The Graduate Center (GC) of the City University of New York
(CUNY) is located in the newly renovated Altman Building at 365 5th
Avenue. The Altman Building occupies a city block bounded by 35th and
34th street on the north and south, respectively, and by Madison
Avenue and 5th Avenue and on the east and west, respectively. The
Altman Building is catty-corner from the Empire State Building. The
GC shares this building with the Science Division of the New York
Public Library and Oxford University Press. The entrance to the GC is
on 5th Avenue between, and almost equidistant from, East 34th Street
and East 35th Street.
The Pennsylvania Station (Amtrak, LLRR and New Jersey
Transit) is between 31st street and 33rd street on 7th Avenue. Grand
Central Station (Metro North) is on 42nd Street at Park Avenue. Park
Avenue is two blocks east of 5th Avenue (Madison is in between). Both
of these main line stations are short walks from the GC. The PATH
trains to New Jersey have a station at 34th Street and 6th Avenue.
There is also a heliport at 34th Street and East river, 6 avenue
blocks to the east.
Almost all the Manhattan subway lines have a station on 34th
Street and within several avenue blocks of the GC. There are a number
of bus lines that run on 34th street and on 5th Avenue. There are a
number of parking garages around the GC. There is money to support
parking for interested students, apply to Steve Cowin
(scccc@cunyvm.cuny,edu).
THE LOCATION OF THE BONE SEMINAR ACTIVITIES WITHIN THE GRADUATE CENTER (GC)
When you enter the GC from 5th Avenue there is a reception
counter in the center of a reception room and the elevators are
straight ahead. If you arrive at the GC with a few minutes to spare
before 7 PM you should consider stopping at the 365 Express on the
1st floor for sandwiches, snacks and drinks and to socialize with the
other seminar attendees and the evening's speaker. The 365 Express is
directly off the GC reception room on the 1st floor (the room you
entered from the street), off the southeast corner of the room. Room
C197 is in the cellar concourse. (For the March seminar, Room 9207 is
on the 9th floor.)
CLOSING NOTES
Future seminars are posted on www.bonenet.net, a website dedicated to
research on the mechanosensory system in bone. (This website is
operational, but not fully developed and suggestions for further
development of the site would be appreciated.) You may request a
reminder for each seminar by sending an email to Steve Cowin
(scccc@cunyvm.cuny.edu).
We will welcome your attendance and hope that you will pass along
this information to interested colleagues. Please direct your
questions, requests for more information and feedback to me.
Kind regards, Steve Cowin
--
************************************
For bone research information, visit .
************************************
PREFERRED MAILING ADDRESS
Stephen C. Cowin
2166 Broadway
Apartment 12D
New York, NY 10024
Phone (212) 799-7970 (Office at Home)
Fax (212) 799-7970 (Office at Home)
Phone (212) 650-5208 (Work)
Fax (212) 650-6727 (Work)
WORK ADDRESS:
Stephen C. Cowin
Director, New York Center for Biomedical Engineering
School of Engineering
The City College
138th Street and Convent Avenue
New York, NY 10031-9198, U. S. A.
*************************************
For information about the New York Center for Biomedical
Engineering visit
*************************************
---------------------------------------------------------------
To unsubscribe send SIGNOFF BIOMCH-L to LISTSERV@nic.surfnet.nl
For information and archives: http://isb.ri.ccf.org/biomch-l
---------------------------------------------------------------