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REGARDING tendon TEM protocol
To: Biomch-L community
I am interested to hear from anyone who has used transmission electron
microscopy to look at cross-sections of tendon. I am working on a project that
is attempting to make measures of fibril diameters and distributions. I
believe that I need help with protocols for fixation and embedding. I have
tried several variations of the usual methods and have gotten less than
satisfactory results. I am aware of some work by Parry and by Oakes in this
area but would be interested to "speak" (e-mail) with someone about the issues
and protocols to get some expert advice on my problem. Any help would be
greatly appreciated. Thanks in advance.
Lou Soslowsky, PhD
University of Michigan
(313)763-9674
lou.soslowsky@med.umich.edu
To: Biomch-L community
I am interested to hear from anyone who has used transmission electron
microscopy to look at cross-sections of tendon. I am working on a project that
is attempting to make measures of fibril diameters and distributions. I
believe that I need help with protocols for fixation and embedding. I have
tried several variations of the usual methods and have gotten less than
satisfactory results. I am aware of some work by Parry and by Oakes in this
area but would be interested to "speak" (e-mail) with someone about the issues
and protocols to get some expert advice on my problem. Any help would be
greatly appreciated. Thanks in advance.
Lou Soslowsky, PhD
University of Michigan
(313)763-9674
lou.soslowsky@med.umich.edu