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Dear Biomch-L subscibers,
I am looking for advice on how to expose cultured cells to very high shear stresses (50 Pa or higher) using a flow chamber or a microfluidic system.
The problem with generating such a high shear stress is that it requires significant pressure (around 10 PSI), which can lead to problems with leaks (if the flow system connections are not locked).
Has anybody tested a similar device? I would be most appreciative if anyone can provide advice on this topic.
thanks in advance,
Oualid Haddad, Ph.D.
Department of Integrative Biology
University of Guelph
Guelph, ON N1G-2W1
Canada
Tel: (519) 824-4120 x58380
http://www.comparativephys.ca/members/members/ohaddad
I am looking for advice on how to expose cultured cells to very high shear stresses (50 Pa or higher) using a flow chamber or a microfluidic system.
The problem with generating such a high shear stress is that it requires significant pressure (around 10 PSI), which can lead to problems with leaks (if the flow system connections are not locked).
Has anybody tested a similar device? I would be most appreciative if anyone can provide advice on this topic.
thanks in advance,
Oualid Haddad, Ph.D.
Department of Integrative Biology
University of Guelph
Guelph, ON N1G-2W1
Canada
Tel: (519) 824-4120 x58380
http://www.comparativephys.ca/members/members/ohaddad