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Effects of freezing on bone specimens

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  • Effects of freezing on bone specimens

    I would like to know if anyone has any information on the effects of
    freezing on the mechanical properties of bone. I vaguely recall this
    topic coming up on biomch-l some time ago but can't remember the details.

    I am interested to find out whether single or multiple freeze/thaw cycles
    affect the mechanical properties of small vertebrae for compression testing.
    Do bones suffer "freezer burn" with subsequent dehydration, even if
    wrapped in polythene cling film? Would it be better to freeze bones in
    small wells of saline?

    Talking of dehydration: If allowed to dehydrate, would the mechanical
    properties of such a bone be restored to normal when rehydrated by submerging
    it in physiological saline?

    I'll post the replies as usual.
    Thanks

    Richard Hillam
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