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Stereology Responses

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  • Stereology Responses

    Here is a listing of the responses I received regarding the stereology
    software. Thanks to all.

    Karl J. Jepsen, Ph.D.
    Department of Orthopaedics
    Case Western Reserve University
    11100 Euclid Ave.
    Cleveland, OH 44106

    We have recently used a SEM based stereo imaging software to quantify
    the surface roughness of human and bovine cortical bone. The preliminary
    results are presented in form the following abstract :

    Vashishth, D. and Bonfield, W., "Aetiology of cortical bone fracture",10th
    conference of the European Society of Biomechanics,Leuven, August 28-31,
    1996, pg. 55.

    The sotware was supplied by Oxford instruments, Analytical systems division,
    (Halifax road, High Wycombe, Bucks,England HP 12 3 SE) and it works in
    conjunction with a link system (an imaging/analysis system for SEM images;
    manufactured by Oxford instruments). If you need any further details, please
    let me know.

    With best wishes

    Deepak Vashishth
    IRC in Biomedical Materials
    Queen Mary and Westfield College
    University of London, UK

    Hi Karl,
    Maria Moalli was showing me a brochure recently from a company called R&M
    Biometrics about a PC program called 'Bioquant', which is capable of doing
    stereology with biological images. Maria is planning on attending a program
    demo here at UofM in the end of October. If you want more info, I can xerox
    the brochures and mail or fax (about 20 pages) them to you.

    One more thing - The Bioquant brochure indicates that the program requires
    an 'add-on' stereology toolkit module in order to use it for this

    Take care,

    Colleen L. Flanagan
    Orthopaedic Research Lab
    University of Michigan

    For image processing and analysis, we use a commercial package called
    Image-Pro Plus (V1.3) by Media Cybernetics. It has several available
    enhancements and an SDK so you can add your own enhancements. We use it
    to measure area fractions of bone and fiber metal in backscatter SEM for
    uncemented hip replacements. We also use it for cortical porosity
    measurements. We just bought it, so I really think we have barely
    exploited all its potentials.

    We got it because we thought our old system might kick the bucket. Our
    old system is a hardware-based Image Technology Corp Model 3000. Its
    software side runs in DOS. I don't know if that company still exists,
    and if so, whether they have a software-based image analysis package.
    You have to feed their black box a video image from somewhere. We use a
    Data Translation DT2851-60 for this purpose and also for camera
    acquisition. An EE masters student wrote a program called Cancellous
    Bone Architecture or CBA. CBA can give us such measurements as bone area,
    bone area density, bone perimeter, intertrabecular area, trabecular
    width, etc. Unfortunately, it's still part of our legacy systems. It's a
    text-mode program, but can be made to run in Windows (same with ITC
    3000). But it's main drawback is it's progammed to use the DT2851-60,
    which prevents us from using any computer with 16M of RAM or greater.


    Ruben V. Igloria, Jr., Engineering Assistant
    Orthopedic Research, Dept of Orthopedic Surgery
    Rush Arthritis and Orthopedics Institute
    Rush-Presbyterian-St. Luke's Medical Center
    2242 W. Harrison St., Suite 103, Chicago, IL 60612-3515