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tissue fixation and mechanical testing

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  • tissue fixation and mechanical testing

    Dear list subscribers,

    We are doing an animal study on the fixation of implants in bone. We are
    interested both in histology and in the mechanical fixation strength, but
    we have a problem in determining both aspects in the same animal. For
    good histology, we need to fix the material as soon as possible after
    obduction. At the moment, we do that in 70% alcohol. However, we have
    serious concerns about changes in the mechanical properties of the
    tissues (and consequently in the fixation strength) due to this. In
    particular, we are concerned about the mechanical property changes in
    cement, fibrous tissue and bone. We could not find studies addressing
    this particular question, and therefore would like to put the following
    questions on the net:

    1. Does anyone have information on the mechanical changes of the cement,
    fibrous tissue and bone due to fixation in alcohol?
    2. Does anyone have a suggestion for an alternative fixation method,
    especially one that would not (or hardly) affect the mechanical

    Obviously, we also considered the possibility of sawing bone and implant
    first and a fixation afterwards, but sawing several slices through an
    implant takes several hours, and our histology department is most
    suspicious about the quality of histology if we would do it like that.
    Maybe there also are suggestions how to reassure these people, e.g. by
    good examples from elsewhere.

    Many thanks in advance,

    Theo Smit
    University Hospital Vrije Universiteit
    Amsterdam, the Netherlands