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Defat cancellous bone

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  • Defat cancellous bone

    Hi,
    I'm currently preparing cubic cancellous bone specimens (2 cm) to test using
    ultrasound (immersion technique). Going through the bibliography I see that
    it is a common practice to remove all fat and marrow from the cavities
    before testing. I put the specimens in ethyl trichloride ulrasound bath for
    4 hours followed by high pressure air jet. The results are fair. I would
    appreciate any respond to the following questions :

    i) Is there a more reliable way to defat cancellous bone ?
    ii) Since many specimens have a closed cell form is it really possible to
    remove all fat ?
    iii) Is there a significant difference in the values of SOS or BUA for
    specimens of cancellous bone with and without marrow ?

    Thank you in advance,

    Dionisis Voulgaris
    Biomedical Engineering Laboratory
    Mechanical Engineering Department
    University of Patras
    Patras, Greece
    E-mail address : voulga@mevax.mech.upatras.gr
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