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Methods for cutting very thin samples of tissue

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  • Methods for cutting very thin samples of tissue

    Hello Bionetters,

    We are having trouble locating a device (if any is available) for
    reproducibility cutting very thin samples of fresh tissue (for example
    meniscus). When I say very thin, I mean 100-150 microns thick. We have
    tried mechanical microtomes, and handheld microtomes, but they do not seem
    to
    work well with very fresh samples. The samples that we are attempting to
    cut must be fresh and cannot be frozen or imbedded in paraffin (which would
    sure make this easy). Just curious whether anyone out there has come across
    this problem and if and how they overcame it.


    Any assistance is appreciated.

    Fabian

    Fabian E. Pollo, Ph.D.
    Director, Motion and Performance Laboratory
    Department of Orthopaedic Surgery
    Baylor University Medical Center
    3500 Gaston Avenue
    Dallas, Texas 75246
    Voice: (214) 820-6300
    Fax: (214)820-1988

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