I am a new PhD student looking for feedback on the best, most accurate and reliable way to measure muscle thickness, fascicle length, and pennation angle change in ultrasound image stacks. I am currently using ImageJ to manually digitise these points on each frame, however the likelihood of human error seems high. Not to mention incredibly time consuming.

If anyone has any tips, tricks, etc. please let me know! Keen to hear what others are doing to be more reliable and accurate.

Thank you